ABSTRACT
Abstract Purpose: To investigate the efficacy of allogeneic mesenchymal stem-cells and autologous mononuclear cells to promote sensorimotor recovery and tissue rescue. Methods: Female rabbits were submitted to the epidural balloon inflation method and the intravenous cells administrations were made after 8 hours or seven days after injury induction. Sensorimotor evaluation of the hindlimbs was performed, and the euthanasia was made thirty days after the treatment. Spinal cords were stained with hematoxylin and eosin. Results: Both therapies given 8 hours after the injury promoted the sensorimotor recovery after a week. Only the group treated after a week with mononuclear cells showed no significant recovery at post-injury day 14. In the days 21 and 28, all treatments promoted significant recovery. Histopathological analysis showed no difference among the experimental groups. Our results showed that both bone marrow-derived cell types promoted significant sensorimotor recovery after injury, and the treatment made at least a week after injury is efficient. Conclusion: The possibilities of therapy with bone marrow-derived cells are large, increasing the therapeutic arsenal for the treatment of spinal cord injury.
Subject(s)
Animals , Female , Rats , Spinal Cord Injuries/surgery , Leukocytes, Mononuclear/transplantation , Bone Marrow Transplantation/methods , Recovery of Function , Mesenchymal Stem Cell Transplantation/methods , Spinal Cord Injuries/physiopathology , Spinal Cord Injuries/pathology , Time Factors , Transplantation, Autologous , Transplantation, Homologous , Tomography, X-Ray Computed , Disease Models, Animal , Neural PathwaysABSTRACT
A cirurgia endoscópica por orifícios naturais é um novo conceito de abordagem endoscópico-cirúrgica, que surge comoalternativa à cirurgia convencional, eliminando incisões abdominais e complicações relacionadas. Ela é realizada pororifícios como estômago, ânus e vagina. A proposta deste estudo foi descrever nova técnica cirúrgica minimamente invasivapara acesso à cavidade abdominal, por NOTES (Natural Orifice Transluminal Endoscopic Surgery) transvaginal em éguas.Foi realizado o acesso através do fórnix vaginal para um inventário da cavidade abdominal em duas éguas. Para tal foirealizada uma sedação prévia com Detomidina intravenosa e lidocaína pela via epidural. O acesso foi feito com auxílio de umtrocater-cânula dorsolateral direita a cérvix; que em seguida foi substituído por um overtube e o endoscópio flexível foiinserido através deste. Foi realizada exploração seriada da cavidade abdominal. O acesso foi realizado facilmente semcomplicações de trans e pós-operatórios. Foi visualizado o antímero direito da cavidade abdominal e suas estruturasanatômicas. O método evidenciou eficiência e mínima invasibilidade para exploração de vísceras abdominais em fêmeasequinas, ficando clara sua grande relevância para diagnóstico de patologias abdominais como seu uso para realização debiópsias.
NOTES, an acronym for Natural Orifice Transluminal Endoscopic Surgery, is a new endoscopic surgery alternative approach which eliminates abdominal incisions and their related complications. NOTES techniques are usually performed through orifices like stomach, anus and vagina. This study aimed the description of a new minimally invasive technique for transvaginal access to abdominal cavity in fowls. Two fowls had its abdominal cavity accessed through their vaginal fórnix. The anesthesia was realized with E.V. Detomidine and lidocaine via epidural. The access was done by a canule-trocater inserted at the right dorso-lateral region of the cervix. The canule was replaced with the aid of an overtube, after what the endoscope was inserted through it. The abdominal cavity was fully inspected.